Unlock the Secrets of the Immunological Synapse: A Comprehensive Guide

Discover the latest methods and techniques for studying the immunological synapse with “The Immunological Synapse – Part B, Volume 178” in the renowned Methods in Cell Biology series. Written by expert author Clment Thomas, this volume provides an in-depth exploration of the cutting-edge approaches for understanding T cell and natural killer (NK) cell synapses.

Imaging and Analysis Techniques

• Uncover the secrets of polarized granule release using Total Internal Reflection Fluorescence (TIRF) microscopy
• Analyze actin reorganization and centrosome polarization for a deeper understanding of cellular dynamics
• Redirect degranulation and quantify interactions between Antigen-Presenting Cells (APCs) and T cells
• Visualize and quantify cytotoxic and chemotactic dynamics using live cell-imaging

Proteomic Analysis and Cellular Interactions

• Delve into the world of proteomic analysis and imaging of NK-tumor cell interactions
• Evaluate natural killer cell effector functions against breast cancer cells derived from human tumor tissue
• Assess the quality of chimeric antigen receptor (CAR)-modified cell immunological synapses using the glass-supported planar lipid bilayer
• Monitor the potency of therapeutic CAR T cells with precision

Viral Titers and Cellular Phagocytosis

• Quantify lymphocytic choriomeningitis virus specific T cells and LCMV viral titers with ease
• Master the murine antibody-dependent cellular phagocytosis assay for enhanced understanding of cellular interactions

This comprehensive volume covers a wide range of methods related to the study of the immunological synapse, providing detailed, step-by-step instructions and essential notes from an international board of leading scientists. Get ready to unlock the secrets of the immunological synapse and take your research to the next level!

Publication Details:

• Publisher: Elsevier Science
• Publication Date: July 27, 2023
• Language: English
• ISBN: 9780443191633 (Hardcover), 9780443191640 (E-book)