Single Biomolecule Detection and Analysis: A Comprehensive Guide

By Tuhin Subhra Santra, this book is a collection of various micro/nanodevice design and fabrication techniques for single-biomolecule detection. It serves as an ideal reference text for graduate students and professionals in diverse subject areas including materials science, biomedical engineering, chemical engineering, mechanical engineering, and nanoscience.

Techniques for Single-Biomolecule Detection

This book discusses techniques of single-biomolecule detection, their advantages, limitations, and applications. It covers comprehensively several electrochemical detection techniques and provides single-molecule separation, sensing, imaging, sequencing, and analysis in detail.

Electrochemical Detection Techniques

Electrochemical detection techniques are one of the key methods discussed in the book. These techniques have several advantages and limitations, which are thoroughly explained along with their applications in various fields.

Cantilever-Based Biomolecule Sensing

The book also examines different types of cantilever-based biomolecule sensing and its limitations. This section provides a detailed understanding of how cantilever-based sensing works and its potential applications.

Micro/Nanotechnologies and Micro/Nanofluidic Devices

Single Biomolecule Detection and Analysis covers single-biomolecule detection and characterization using micro/nanotechnologies and micro/nanofluidic devices. These technologies have revolutionized the field of biomolecule detection by providing highly sensitive and specific methods for detection and analysis.

Electrical and Magnetic Detection Technologies

The book discusses electrical and magnetic detection technologies, which are crucial for detecting and analyzing biomolecules at the single-molecule level. These technologies have several applications in biomedical engineering, materials science, and nanoscience.

Microscopy and Spectroscopy Techniques

Microscopy and spectroscopy techniques are also covered in the book. These techniques are essential for single-biomolecule detection, analysis, and their biomedical engineering applications. They provide a detailed understanding of the structure and properties of biomolecules.

Key Applications and Techniques

Single Biomolecule Detection and Analysis covers key important biosensors-based detection, stochastic optical reconstruction microscopy-based detection, electrochemical detection, metabolic engineering of animal cells, single-molecule intracellular delivery and tracking, terahertz spectroscopy-based detection, total internal reflection fluorescence (TIFR) detection, and Fluorescence Correlation Spectroscopy (FCS) detection.

Biosensors-Based Detection

Biosensors-based detection is one of the key applications discussed in the book. Biosensors are devices that use biological molecules or tissues to detect specific substances and produce a digital electronic signal proportional to the concentration of the substance.

Stochastic Optical Reconstruction Microscopy-Based Detection

Stochastic optical reconstruction microscopy (STORM) is another technique covered in the book. STORM is a super-resolution imaging technique that allows for the imaging of biomolecules at the nanoscale.

Electrochemical Detection

Electrochemical detection is a widely used technique for detecting biomolecules. The book provides a comprehensive understanding of electrochemical detection, its advantages, limitations, and applications.

Metabolic Engineering of Animal Cells

Metabolic engineering of animal cells is a technique used to modify cellular metabolism for a specific purpose. The book discusses the application of this technique in single-biomolecule detection and analysis.

Single-Molecule Intracellular Delivery and Tracking

Single-molecule intracellular delivery and tracking is a crucial technique for understanding the behavior of biomolecules within cells. The book covers this technique in detail, including its advantages and limitations.

Terahertz Spectroscopy-Based Detection

Terahertz spectroscopy is a technique used to detect and analyze biomolecules using terahertz radiation. The book provides an in-depth understanding of this technique and its applications.

Total Internal Reflection Fluorescence Detection

Total internal reflection fluorescence (TIRF) detection is a technique used to detect biomolecules near the surface of a material. The book discusses the principles and applications of TIRF detection.

Fluorescence Correlation Spectroscopy Detection

Fluorescence correlation spectroscopy (FCS) is a technique used to detect and analyze biomolecules using the fluctuations in fluorescence emitted by labeled molecules. The book covers the principles and applications of FCS detection.

Conclusion

Single Biomolecule Detection and Analysis is a comprehensive guide for graduate students and professionals in diverse subject areas including materials science, biomedical engineering, chemical engineering, mechanical engineering, and nanoscience. It covers key techniques, applications, and limitations of single-biomolecule detection and analysis, providing a detailed understanding of the field and its potential applications.