Structure-Function Toolbox for Membrane Transporter and Channels

Volume 594 of the Methods in Enzymology series, edited by Christine Ziegler, offers a comprehensive guide to the latest techniques and methodologies in the field of membrane transporters and channels. This volume continues the legacy of the premier serial with high-quality chapters authored by leading experts in the field.

Key Techniques and Methodologies

This updated serial includes a range of cutting-edge chapters, such as:

• Cryo-EM on membrane proteins embedded in nanodics, providing insights into the structure and function of membrane proteins at the nanoscale.
• Solid-Supported membrane-based electrophysiology on membrane transporters and channels, offering a novel approach to studying the electrical properties of membrane proteins.
• Saposin-lipoprotein scaffolds for structure determination of membrane transporters and channels, detailing a new method for stabilizing and crystallizing membrane proteins for structural analysis.
• Single-molecule FRET on transporters, showcasing a technique for investigating the dynamics and conformational changes of individual transporter molecules.
• Dynamics of channels and transporters investigated by NMR, highlighting the use of nuclear magnetic resonance spectroscopy to probe the structure and dynamics of membrane proteins.
• Structure-function studies on channels and transporters, providing an overview of various approaches to understanding the relationship between the structure and function of these proteins.
• MemStar, a new GFP-based expression and purification system for transporters and channels, introducing a novel tool for the high-throughput expression, purification, and analysis of membrane proteins.

Product Details

Series: Methods in Enzymology (Book 594)
Format: Hardcover
Pages: 302
Publisher: Academic Press; 1 edition (August 18, 2017)
Language: English
ISBN-10: 0128123532
ISBN-13: 978-0128123539
Product Dimensions: 0.8 x 6 x 9 inches