Comprehensive Guide to Human Pluripotent Stem Cells

Human Pluripotent Stem Cells: A Practical Guide is a comprehensive book that covers the entire induced pluripotent stem cell basic work flow. It is developed from a training course and offers in-depth information on all aspects of pluripotent stem cells (PSCs), including their functions, culture, reprogramming, differentiation, and characterization.

Introduction to Stem Cells

The book begins with an introduction to stem cells, providing readers with a solid foundation of knowledge on the subject. It explains the unique properties of PSCs, such as their ability to divide indefinitely, self-renew, and differentiate into various cell types in the normal developmental pathway.

Feeder-Dependent and Feeder-Free Culture of hESC and hiPSC

The guide demonstrates the methods for culturing human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) using both feeder-dependent and feeder-free techniques. These methods are crucial for maintaining the pluripotency and ensuring the quality of PSCs for research and therapeutic applications.

Reprogramming and Differentiation

Human Pluripotent Stem Cells: A Practical Guide also covers the reprogramming of adult cells into PSCs and the differentiation of PSCs into specific cell types. These processes are critical for understanding the potential of PSCs in regenerative medicine and tissue engineering.

Characterization of PSCs

The book addresses the methods commonly used to determine the pluripotency of PSCs, including the expression of self-renewal markers and the assessment of differentiation potential. These characterization methods are essential for verifying the quality and functionality of PSCs.

Appendices

The guide includes four appendixes that provide valuable resources for researchers, including information on reagents, media, and solutions; common antibodies; consumables and equipment; and logs and forms. These appendices make the book an indispensable reference for both novice and experienced stem cell researchers.

Expert Insights and Visual Aids

Written by experts with extensive experience in stem cell technologies, Human Pluripotent Stem Cells: A Practical Guide offers helpful tips and tricks that are normally omitted from regular research papers. The book also features useful images and diagrammatic illustrations to support the technical aspects and results visually, making it easier for readers to understand complex concepts and techniques.

Target Audience

This comprehensive guide is ideal for stem cell researchers, including principal investigators, and others in university and industry settings. It is also suitable for new graduate students in PSC labs who need a thorough understanding of the principles and practices of working with human pluripotent stem cells.

Publication Details

Human Pluripotent Stem Cells: A Practical Guide is published by Wiley-Blackwell and is available in hardcover format. It consists of 176 pages and was published on February 13, 2018. The ISBN-10 is 1119394333, and the ISBN-13 is 978-1119394334.