Understanding Monoclonal Antibodies: A Comprehensive Guide

By Roohi Bansal

Introduction to Monoclonal Antibodies

Monoclonal antibodies (mAbs) have revolutionized the fields of therapeutics and diagnostics. This book aims to provide a detailed understanding of the structure, types, functions, and gene organization of antibodies, with a special focus on mAbs.

Generation of Monoclonal Antibodies

The book describes the different ways of generating chimeric, humanized, and fully human monoclonal antibodies. It emphasizes phage display, hybridoma, and rDNA technology as the primary methods for generating these antibodies.

Recombinant Antibody Formats

The book focuses on various recombinant antibody formats in detail, including:

• Drug conjugates: Antibody-drug conjugates (ADCs), Immunotoxins (Recombinant, Humanized and Fully Human) and Antibody-antibiotic conjugate (AAC).
• Bispecific antibodies: scFv based (BiTE, DARTs and TandAbs) and Full-length IgG based.
• Abzymes and Antibody-directed enzyme prodrug therapy (ADEPT).
• Fc-fusion proteins.
• Single-domain antibodies (VHH and IgNAR sdAb).

Therapeutic Applications of Monoclonal Antibodies

The book discusses the various therapeutic applications of monoclonal antibodies, along with the immunogenicity issues. It also covers the modes of administration and side effects of monoclonal antibodies, as well as the challenges and issues faced while developing a monoclonal antibody into a therapeutic agent.

Modifications to Decrease Immunogenicity and Increase Efficacy

Modifications introduced by researchers to decrease the immunogenicity issues and increase the efficacy of therapeutic mAbs are also described in the book.

Target Audience

This book is an invaluable resource for researchers and students in biology and medicine, biotechnology, immunology, genetics, molecular biology, and anyone interested in antibody engineering.

Product Details

Author: Roohi Bansal

Publication Date: December 4, 2021

Language: English

Pages: 387

ISBN-10: 9355781601

ISBN-13: 978-9355781604