Freshney’s Culture of Animal Cells: The Definitive Guide to Cell and Tissue Culture

Get ready to dive into the most comprehensive and up-to-date resource on the principles, techniques, equipment, and applications of cell and tissue culture. The eighth edition of Freshney’s Culture of Animal Cells is a must-have for anyone involved in the field, covering everything from the biology of cultured cells to the latest emerging applications.

What’s New in the Eighth Edition

This extensively revised edition reflects the latest techniques and emerging applications in cell culture, including:

• The use of CRISPR/Cas9 for gene editing
• The adoption of chemically defined conditions for stem cell culture
• A brand-new chapter on the origin and evolution of cell lines
• A dedicated chapter on irreproducible research, its causes, and the importance of reproducibility and good cell culture practice

Comprehensive Coverage of Cell Culture Topics

This landmark text provides single-volume coverage of:

• Basic skills and protocols
• Specialized techniques and applications
• New and emerging developments in the field

Covering every essential area of animal cell culture, including:

• Lab design and disaster contingency planning
• Safety and bioethics
• Media preparation and primary culture
• Mycoplasma and authentication testing
• Cell line characterization and cryopreservation
• Training and troubleshooting

New Content and Online Resources

The eighth edition features a wealth of new content, including protocols for:

• Gene delivery
• iPSC generation and culture
• Tumor spheroid formation

An updated and expanded companion website contains figures, artwork, and supplementary protocols to download and print, making it an indispensable resource for researchers and students alike.

With its comprehensive coverage and cutting-edge content, Freshney’s Culture of Animal Cells is the ultimate guide for anyone involved in cell biology, molecular biology, and genetics laboratories.