Unlock the Secrets of the Immunological Synapse with Volume 178 of Methods in Cell Biology

Edited by renowned expert Clment Thomas, The Immunological Synapse – Part B, Volume 178 is a cutting-edge resource for scientists and researchers seeking to advance their understanding of the immunological synapse. This comprehensive volume is part of the esteemed Methods in Cell Biology series, offering a state-of-the-art exploration of T cell and natural killer (NK) cell synapses.

Groundbreaking Methods for Immunological Synapse Research

This authoritative volume covers a range of innovative methods and techniques, including:

• Imaging polarized granule release using TIRF microscopy
• Analysis of actin reorganization and centrosome polarization
• Redirected degranulation
• Live cell-imaging to quantify cytotoxic and chemotactic dynamics
• Quantification of interactions between APCs and T cells
• Assessment of membrane lipid state at the immunological synapse
• Proteomic analysis and imaging of NK-tumor cell interaction
• Evaluating natural killer cell effector functions against breast cancer cells derived from human tumor tissue
• Evaluation of chimeric antigen receptor (CAR)-modified cell immunological synapse quality using the glass-supported planar lipid bilayer
• Monitoring potency of therapeutic CAR T cells
• Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers
• The murine antibody-dependent cellular phagocytosis assay

Each chapter provides detailed, step-by-step methods, as well as valuable notes and insights from an international board of leading scientists.

A Valuable Resource for Immunology Researchers

The Immunological Synapse – Part B, Volume 178 is an indispensable reference for researchers, students, and professionals in the field of immunology, cell biology, and related disciplines. With its comprehensive coverage of cutting-edge methods and techniques, this volume is poised to become a go-to resource for those seeking to advance their understanding of the immunological synapse.

Published by Elsevier Science, this volume is available in English and features ISBN numbers 9780443191633 and 9780443191640.